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small areas of 4 μm × 4 μm regions on the surface of living ibroblasts (Balb
3T3) using a colloidal probe that was coated with ibronectin searching for
its speciic receptor, integrins. First, the data showed reproducible mapping
patterns between consecutive maps
(
Fig. 12.2a
)
but with a gradual change of
the mapping pattern with time. Then, the unbinding work was measured over
a wide area of a living cell by repeating the measurement each time shifting
the scanning area
(
Fig. 12.2b
)
.
(a)
(b)
Figure 12.2.
(a) Six time lapse mapping results of the same area obtained on a living
Balb/3T3 ibroblast cell by scanning with an AFM probe coated with ibronectin every
1 minute from 1 to 4. Horizontal scales are in micrometre and the vertical axis is the
separation work in 10
J/μm
2
. (b) Composite mapping results on a living cell. The
framed area on the right image (phase contrast) was divided into several sub-areas
and each area was scanned with ibronectin-coated probe. The results from multiple
areas were assembled to create the composite map on the left. Reproduced with
permission from Kim
et al.
39
17
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