Biology Reference
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individual luorescent spots. 71 Improved antenna geometries by carefully
reducing the width and adjusting the length to optimum resonance in liquid
conditions should lead to true live cell imaging below 10 nm resolution with
position accuracy in the sub-nanometric range.
(a)
(b)
Figure 9.7. (a) Different antenna probes fabricated using FIB milling. (b) Super-
resolution image of LFA-1 on monocytes in liquid conditions obtained with an antenna
probe. Image adapted with permission from Ref. 71. © (2010) Wiley-VHC.
Aside from these developments in nanophotonics to increase further
the resolution of NSOM, there is also growing interest in incorporating
into NSOM other capabilities, which have been recently reported for AFM.
In particular, it will be extremely appealing to implement the molecular
recognition technique (TREC) in NSOMs. TREC, which has been introduced
by the Hinterdorfer group a few years ago,
involves the use of chemically
functionalized tips to identify and localize speciic molecules on the cell
membrane during scanning.
22
When combined with NSOM, one could in
principle obtain simultaneous topography, molecular recognition and optical
imaging at the nanometre scale and in one single measurement.
72
 
 
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