Biology Reference
In-Depth Information
“turquoise” and the membrane proteins are shown in “brown”. Membrane
fragmentation occurs frequently because of the preparation method we used.
The broken line in the upper part of Fig. 6.2 corresponds to the proile line in
the lower part showing the lipid bilayer with a height of about 5 nm. Proteins
appear with different shapes and protrude from the inner surface of the
plasma membrane with heights up to 20 nm.
(a)
(b)
Figure 6.2. (a) Colour-coded view of a 9 μm 2 AFM scan area containing plasma
membrane fragments attached to the poly--lysine-coated glass surface. Poly--lysine-
coated glass is shown in “blue”, the lipid bilayer membrane is shown in “turquoise”
and the membrane proteins are shown in “brown”. (b) The red line in Fig. 6.2a
corresponds to the proile line in 6.2b. The section line shows three height levels: (1)
the poly--lysine-coated glass surface, (2) the lipid bilayer with a height of about 5 nm
and (3) proteins protruding from the inner surface of the plasma membrane with a
height up to 20 nm. 3
The main difference between cAMP-stimulated and non-stimulated oocyte
membrane is the protein density. Quantiication of protein distribution is
shown in Fig. 6.3 . Molecular volumes were estimated from protein heights
measured by AFM. Molecular weights were then calculated from the
respective volume measurements.
 
 
 
Search WWH ::




Custom Search