RESOLVING THE HIGH-RESOLUTION ARCHITECTURE, ASSEMBLY AND FUNCTIONAL REPERTOIRE OF BACTERIAL SYSTEMS BY IN VITRO ATOMIC FORCE MICROSCOPY - Life at the Nanoscale: Atomic Force Microscopy of Live Cells - page 93

dynamic environmental and chemical probing of microbial surface reactions.
Further incorporation of AFM-based immunolabelling techniques could allow
the identiication of spore coat proteins that play a role in spore germination
and provide a structural understanding of how these proteins regulate spore
survival, germination and disease.

Acknowledgements

The author thanks M. Plomp for his critical contributions to this work
and B. Vogelstein, T.J. Leighton, P. Setlow and H.-Y. Holman for spore and
bacteria preparations, helpful discussions and encouragement. This work
was performed under the auspices of the US Department of Energy by
Lawrence Livermore National Laboratory under contract number DE-AC52-
07NA27344. This work was supported by the Lawrence Livermore National
Laboratory through Laboratory Directed Research and Development Grants
04-ERD-002 and 08-LW-027.

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