Biological Applications of Dynamic Force Spectroscopy - Dynamic Force Spectroscopy and Biomolecular Recognition - page 224

TABLE 6.5
DFS Results for a Collection of Complexes or Systems Involved in Adhesion Processes: Unbinding Force at a Given Loading
Rate (in Parentheses); Dissociation Rate

k off and Energy Barrier Width x

β (in Parentheses) as Extracted by Applying the

Bell-Evans Model for Each Linear Trend Observed in the F* versus ln( r F ) Plot (see Equation 6.2)

System: Immobilization: Cantilever Unbinding Loading

s − 1 Substrate/ Substrate/ spring Force (pN) rate k off ( ) Reference

Tip Tip constant (Loading range ( x

β (nm))

k

cant (N/m)

Rate (nN/s)) (nN/s)

P-selectin/ Glass 0.06 120 (15) 12-240 0.022 (0.25) Fritz

P-selectin ligand Si 3 N 4 et al., 1998

VE Cadherin/ Glass (PEG) 0.03 32 (10) 10-120 1.8 (0.59) Baumgartner

VE Cadherin Si 3 N 4 (PEG) et al., 2000

Platelet / Glass 0.06 80 (12) 10-50 22 (0.1) Lee and

Marchant

RGD-peptide Si 3 N 4 2001

P-selectin/ Culture disk (cell) 0.01-0.04 150 (1) 0.1-10 0.2 (0.14) Hanley

P-selectin ligand Si 3 N 4 et al., 2003

10 − 5 -0.033

Fibronectin (wt)/ Culture disk 60 (1) 0.02-50 0.13 (0.4) Li

α 5 β 1 Integrin Si 3 N 4 (cell) 33.5(0.09) et al. 2003

Fibronectin (mut.)/ 0.012 (0.44)

α 5 β 1 Integrin 29.1(0.91)

VCAM-1 Culture disk 0.01 50 (0.8) 0.1-100 59 (0.1) Zhang

α 4 β 1 Integrin Si 3 N 4 0.13 (0.59) et al., 2004

P-Selectin/ Glass (lipid) 0.004-0.013 20 (1) 3-300 0.077 (2.41) Marshall

P-selectin ligand Si 3 N 4 33.6 (0.099) et al., 2005

N-Cadherin/ Culture disk (cell) 0.01 30 (1) 0.05-10 0.98 (0.77) Panorchan

N-Cadherin Si 3 N 4 (cell) et al., 2006

E-Cadherin/ Culture disk (cell) 73 (1) 1.09 (0.32)

E-Cadherin Si 3 N 4 (cell) 4.00 (0.10)

Erythrocyte / Glass 0.019 73 (1) 1-30 0.023 (0.17) Carvalho

Fibrinogen Si 3 N 4 60 (1) 60.9 (0.17) et al., 2010

Platelet/ 0.034 (0.072)