Biomedical Engineering Reference
In-Depth Information
TABLE 6.4
DFS Results for a Collection of DNA-basedComplexes Unbinding Force at a Given Loading Rate (in parentheses);Dissociation
Rate
k
off
and Energy Barrier Width
x
β
(in parentheses) as Extracted by Applying the Bell-Evans Model for Each Linear Trend
Observed in the
F*
versus ln(
r
F
) plot (see Equation 6.2)
System:
Immobilization:
Cantilever
Unbinding
Loading
s
−
1
Substrate/
Substrate/
Spring
Force (pN)
Rate
k
off
(
)
Reference
Tip
Tip
Constant
(Loading
Range
(
x
β
(nm))
k
cant
(N/m)
Rate (nN/s))
(nN/s)
10
−
2
DNA strand 10 bp
Glass (PEG)
0.012-0.017
20(1)
0.02-10
(1.0)
Strunz
10
−
6
DNAstrand20bp
Si
3
N
4
(PEG)
48 (1)
(1.4)
et al., 1999
10
−
10
DNA strand 30 bp
42(1)
(2.0)
11
◦
C (0.18)
DNA strand 16 bp
Glass (PEG)
0.02
0.05-10
T
=
Schumakovitch
=
27
◦
C46(1)
=
27
◦
C 0.5 (0.4)
Si
3
N
4
(PEG)
T
T
et al., 2002
36
◦
C 0.5 (0.9)
T
=
Lex A repressor/
Aminoslide
0.008-0.018
NA
0.5-9
3.1 (5.4)
Kuhner
DNA (rec A)
Si
3
N
4
et al., 2004
DNA fragment/
Mica
NA
Wild-type NA
0.09-100
3.1 (0.68)
Eckel
peptide
Si
3
N
4
(PEG)
mut1 61 (1)
0.071 (0.93)
et al., 2003
mut2 55 (1)
49.5 (0.72)
DNA aptamers/
Silicon
0.06-0.12
86-145 (NA)
80-210
NA
Jiang et al.,
Immunoglobulin E
Si
3
N
4
2003
DNA fragment/
Mica
0.04-0.07
2-500
38 (0.18)
Krasnoslobodtsev
Sfil protein/
Si
3
N
4
100 (1)
70 (0.19)
et al., 2007
248 (0.19)
Note
: Some details of the experimental setup: the immobilization strategies of the biomolecules to both the substrate and the AFM tip; the cantilever spring constant;
the loading rate range. (NA = not available).
Search WWH ::
Custom Search