Biomedical Engineering Reference
In-Depth Information
TABLE 6.2
DFS Results for a Collection of Antigen-Antibody Complexes: Unbinding Force at a Given Loading Rate (in parentheses);
Dissociation Rate
k
off
and Energy Barrier Width
x
β
(in parentheses) as Extracted by Applying the Bell-Evans Model for Each
Linear Trend Observed in the
F*
versus ln(
r
F
) plot (see Equation 6.2)
System:
Immobilization:
Cantilever
Unbinding
Loading
s
−
1
Substrate/
Substrate/
Spring
Force (pN)
Rate
k
off
(
)
Reference
Tip
Tip
Constant
(Loading
Range
(
x
β
(
nm))
k
cant
(N/m)
Rate (nN/s))
(nN/s)
10
−
5
HSA/
Mica (PEG)
0.27
244 (1)
0.1-5
1
.
7
·
(0.5)
Hinterdorfer
Anti-HSA
Si
3
N
4
(PEG)
et al., 1996
Antiferritin/
Silicon (Silane)
0.043-0.057
50 (NA)
NA
NA
Allen
Ferritin
Si
3
N
4
et al., 1997
Antifluorescein/
Gold
0.008-0.014
140(1)
0.1-10
0.003 (0.4)
Schwesinger
Fluorescein
Si
3
N
4
2.1 (0.05)
et al., 2000
Antilysozime/
Gold
0.01
50 (1)
1-100
0.001 (1)
Berquand
Lysozime
Gold-coated
150 (0.06)
et al., 2005
2.6 10
−
3
Mucin 1/
Gold
0.04-0.18
120 (1)
0.4-10
(2.8)
Sulchek
Anti Mucin 1
Gold-coated (PEG)
et al., 2005
ICAM-1/
Culture disk
0.004-0.013
100 (1)
0.05-100
0.55 (2.6)
Wojcikiewicz
LF Antigen 1
Si
3
N
4
(Cells)
19 (0.49)
et al., 2006
ICAM-2/
60 (1)
0.05-100
0.31 (4.5)
LF Antigen 1
0.015 (1.15)
Anti-digoxigenin/
Glass (PEG)
0.006-0.05
40 (2)
5-100
0.015 (1.15)
Neuert
Digoxigenin
Si
3
N
4
(PEG)
4.56 (0.35)
et al., 2006
Anti-GCN4/
Glass (PEG)
0.06-0.08
50(1.5)
0.081-40
3.9 10
−
3
(0.88)
Morfill
4.9 10
−
4
GCN4
Si
3
N
4
(PEG)
(0.90)
et al., 2007
8.2 10
−
4
(0.92)
Note
: Some details of the experimental setup: the immobilization strategies of the biomolecules to both the substrate and the AFM tip; the cantilever spring constant;
the loading rate range. (NA = not available).
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