Biomedical Engineering Reference
In-Depth Information
TABLE 6.1
DFS Results for a Collection of Avidin-Biotin and Streptavidin-Biotin Pairs: Unbinding Force at a Given Loading Rate (in
parentheses); Dissociation Rate
k
off
and Energy Barrier Width
x
β
(in parentheses) as Extracted by Applying the Bell-Evans
Model for Each Linear Trend Observed in the
F*
versus ln(
r
F
) Plot (see Equation 6.2)
System:
Immobilization:
Cantilever
Unbinding
Loading
s
−
1
Substrate/
Substrate/
Spring
Force (pN)
Rate
k
off
(
)
Reference
Tip
Tip
Constant
(Loading
Range
(
x
β
(nm))
k
cant
(N/m)
Rate (nN/s))
(nN/s)
Biotin-BSA/
Agarose bead
0.06
160 (NA)
NA
NA
Florin
Avidin
Si
3
N
4
et al., 1994
10
−
3
Avidin/
Gold (Biotin)
0.034
38 (1)
0.01-10
(2)
De Paris
Biotin
Si
3
N
4
(PEG)
100 (0.3)
et al., 2000
10
−
5
Biotin-BSA/
Agarose bead
0.01-0.05
120 (1)
0.1-5
1
.
71
·
(0.5)
Yuan
Streptavidin
Si
3
N
4
2.1 (0.05)
et al., 2000
Streptavidin/
Silicon
0.12
83 (1)
0.1-1000
5.6 (0.12)
William
Biotin-BSA
Si
3
N
4
et al., 2000
Biotin-BSA/
Glass
0.039
167 (30)
30-6000
NA
Lo
Streptavidin
Si
3
N
4
et al., 2001
Streptavidin/
Silicon (Silane)
0.01-0.09
50 (1)
1-100
0.2 (0.6)
Patel
Biotin-BSA
Si
3
N
4
69 (0.14)
et al., 2004
Biotin-BSA/
Glass (Silane)
0.01-0.4
50 (1)
0.3-9.6
0.56 (0.081)
de Odrowaz
Streptavidin
Si
3
N
4
(Silane)
2.98 (0.024)
et al., 2006
Biotin-BSA/
Agarose bead
0.01
50 (1)
0.03-200
0.1 (0.38)
Rico and Moy,
Streptavidin
Si
3
N
4
(Biotin)
23 (0.09)
2007
Streptavidin/
Glass (PEG)
0.05-0.16
50(1)
0.1-100
2.86 (0.28)
Guo
Biotin
Si
3
N
4
(PEG)
78 (0.073)
et al., 2008
10
−
7
Streptavidin/
Mica
0.01
15-25 (0.2)
0.1-2
2
.
1
·
(0.1)
Walton
Biotin-BSA
Si
3
N
4
(Silane)
2.1 (0.05)
et al., 2008
Streptavidin/
Gold
0.03-0.32
68 (1.2)
1.8-960
0.07 (0.40)
Teulon
Biotin
Gold (PEG)
4.38 (0.08)
et al., 2011
Note
: Some details of the experimental setup: the immobilization strategies of the biomolecules to both the substrate and the AFM tip; the cantilever spring constant;
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