Biomolecular Recognition: Analysis of DFS Data - Dynamic Force Spectroscopy and Biomolecular Recognition - page 163

5 Biomolecular Recognition:

Analysis of DFS Data

Michael Odorico, Jean-Marie Teulon,
Yannick Delcuze, Shu-wen W. Chen,
Pierre Parot, and Jean-Luc Pellequer

CONTENTS
5.1 Introduction to Dynamic Force Spectroscopy .............................................. 163
5.2 Energy Landscape and Single Molecules ..................................................... 167
5.3 Single and Multiple Bonds ........................................................................... 168
5.4 Specific and Nonspecific Interactions........................................................... 170
5.5 Loading Rate................................................................................................. 173
5.6 Detecting Rupture Events ............................................................................. 174
5.7 CPD and Most Probable Rupture Forces...................................................... 177
5.8 Single and Multiple Slopes in Bell-Evans Plots ........................................... 178
5.9 Multiple Parallel Unbinding in Bell-Evans Plots ......................................... 181
5.10 Existing Software and Methods for DFS Data Analysis .............................. 183
5.11 Conclusions and Perspectives ....................................................................... 185
Acknowledgments.................................................................................................. 186
Abbreviations ......................................................................................................... 186
References.............................................................................................................. 187

5.1 INTRODUCTION TO DYNAMIC FORCE SPECTROSCOPY
One of the first meanings of dynamic force spectroscopy (DFS) refers to analyzing
the dynamic interaction of the scanning force microscope tip with the sample surface.
To our knowledge, this expression first appeared in the literature from the group of
Harald Fuchs (Anczykowski et al. 1996) followed by a dissertation, Kraftspectro-
scopie an einzelnen Molek ulen of Matthias Rief, in Munich on 1997. Afterward, the
term was reused by the group of Evan Evans (Evans & Ritchie, 1997; Merkel et al.
1999) to probe the complex relation between force-lifetime and chemistry in single
molecular bonds .Thefirst review on the topic was published in 2000 (Janshoff et al.
2000) and the acronym DFS appeared in 2001 (Evans, 2001). Now, more than 100
publications directly refer to DFS in their title.
In DFS, the word spectroscopy , despite its widely use, is a misleading term
because, unlike other true spectroscopy, there is no matter-radiation interaction
involved in DFS experiments. DFS mainly consists in measuring the behavior of

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