Biomedical Engineering Reference
In-Depth Information
TABLE 4.3
Examples of Compounds for Functionalization of Silanols
Coupling Groups
Name of Compound
Chemical Formula
Amine to amine
Disuccinimidyl
glutarate
O
O
O
O
(DSG)
O
N
N
O
O
O
Glutaraldehyde
a
O
O
H
H
NHS-PEG-NHS
b
O
O
O
O
O
NO
O
N
n
O
O
NHS-PEG-NH-FMOC
c
Amine and create protected
amine
O
O
N
O
O
N
O
n
O
O
Amine to thiol
N-[γ-maleimidobutyryloxy]
succinimide ester
O
O
O
N
N
O
O
O
NHS-PEG-Mal
b
O
O
O
N
O
N
NO
n
O
O
O
Amine to avidin (or strepta-
vidin)
NHS-PEG-Biotin
O
O
N
S
O
N
O
n
O
O
HN
NH
O
a
Coupling is followed by the Schiff base reduction with NaCNBH
3
, see Figure 4.5.
b
Various tether lengths are commercially available, from several ethoxy monomer groups in the linker
chain to long polymers with number of monomers ranging from
200.
c
This compound can be used to move amine functional group away from the surface. After reacting with
the surface amines, FMOC protection can be removed (20%-50% piperidine in DMF) exposing
PEG-tethered amines.
∼
25 to
∼
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