Effects of the cultivation of genetically modified Bt crops on nontarget soil organisms - Microbial Ecology in Sustainable Agroecosystems - page 169

Table 8.3 Summary of Effects of Cultivation of Bt Crops or Amendment with Bt Proteins on Soil Bacteria (continued)
Organism/activity
tested

Bt crop plant/
experimental variable Methodology Study location Protein Effect on bacteria Source

Soil microbial
community

T-RFLP Field Litterbags containing

straw or roots from Bt
(KMD) and non- Bt
( XiuShui 11) rice

Cry1Ab No negative effect of

Bt rice residues on
soil bacteria
community
composition

Lu et al.
(2010a,
2010b)

Rhizosphere bacterial
community structure

PCR-amplified
16S rRNA genes

Field Soil with Bt (Event

MON88017) and three
non- Bt maize lines

Cry3Bb1 No negative effect of

Bt maize on
rhizosphere bacterial
community

Miethling-
Graff et al.
(2010)

Soil microbial
community structure

DGGE
Sequences of the
16S rRNA genes

Greenhouse Soil with two Bt

(Events MON810 and
Nongda 1246*1428)
and their non- Bt
maize isolines
(Pioneer 34B23 and
Nongda 3138)
Soil amended with Bt
and non- Bt maize
leaves and stalks

Cry1Ab,
Cry1A

Neither actively
growing Bt maize nor
Bt maize residue had
a negative effect on
bacterial community
structure

Tan et al.
(2010)

Soil bacteria: Azotobacter
Denitrifying bacteria
Ammonia-oxidizing
bacteria
Microbial diversity

Selective plating
MPN

Field Soil with Bt cotton and

non- Bt cotton

Cry1Ac
and/or
CpTI
protein

No significant
difference in number
of each microbial
population or
diversity indices
between Bt or non- Bt
cotton

Li et al. (2011)

(continued)