Biomedical Engineering Reference
In-Depth Information
d
n
4
y
3
n
g
|
3
Figure 4.5
Tumor targeted gene transfection.
hyaluronic acid (HA), and then coated the cationic surface of PEI-PBLG/
DNA complexes via electrostatic interaction.
33
The resulting HA-RGD/PEI-
PBLG/DNA ternary complexes showed higher cell viability and transfection
efficiency compared with PEI-PBLG/DNA, owing to the RGD target bonding
affinity for integrin on HeLa cells.
HA is a biodegradable, non-immunogenic, non-cytotoxic, and negatively
charged polysaccharide. It has several advantages, including the negligible
nonspecific interaction with serum components due to the polyanionic
characteristics and the highly efficient target specific delivery to the liver
tissues with HA receptors. Yao et al. synthesized HA-g-PEI copolymer by an
imine reaction between periodate-oxidized HA and PEI.
34
This compound
Table 4.1
Ligands used to target tumors and enhance transfection efficiency
of PEI-based vectors.
Ligand
Target
Ref.
RGD
HepG2, HeLa
33
Hyaluronic acid
Liver and kidney cancer cells
34
Folate
SKOV3, HeLa
35,36
Tetanus toxin fragment
Neuronal cell lines
37
Rabies virus glycoprotein
Mouse neuroblastoma Neuro2a cells
38
Chondrocyte-homing peptide
Chondrocyte cells
39
Avidin
HepG2
40-42
Transferrin
HepG2, HeLa
43
N-Acetylglucosamine
293FT, HeLa
44
Phenylboronic acid
HepG2
45
