Biomedical Engineering Reference
In-Depth Information
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Figure 8.4
Dectin-1 expression on thio-Mws and cellular uptake of DNA/SPG
complexes due to dectin-1. Pre-culturing time dependence of the amount
of mRNA expression expressed in thio-Mws. After seeding of 1.0 610
6
cells/well in six-well plates, the levels of dectin-1 and GAPDH were
assayed by RT-PCR. Data represent the mean ¡ SD of three
experiments. (B) Flow cytometry analysis profiles for 0 h (dashed line)
and 24 h pre-cultured thio-Mws(shaded in gray) after anti-dectin-1
antibody staining. The solid line histogram shows unstained control. (C)
Comparison of the FITC fluorescence intensity from the cell lysates,
after FITC-ODN was applied to the different pre-culturing cells: 3 h
(white), 24 h (black), or 48 h (gray) with or without complexation. Data
represent the mean ¡ SD of triplicate wells. (D) Fluorescence images for
FITC and TAMRA after TAMRA-ODN was applied with or without
complexation with FITC-SPG to 48 h pre-cultured thio-Mws. The bar
represents 10 mm.
thio-Mws, and the amount of secreted IL-12 was measured with ELISA
(Figure 8.5).
48
The naked CpG-dA
60
produced low levels of IL-12
(0.8 ng mL
21
) in macrophages, and this value was consistent with other
results.
49
In the range of [mG]/[dA] 5 021.0, the production slightly increased
with an increase of the amount of SPG, and dramatically enhanced at [mG]/
