Biomedical Engineering Reference
In-Depth Information
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Figure 8.2
(A) SEC chromatograms for tSPG (M
w
5 4.5 6 10
5
), dA
60
, and dA
60
/SPG
prepared at the stoichiometric composition. From the top, the weight-
averaged molecular weight, the scattering intensity at 90u, the refractive index
signal, and the UV absorbance at 260 nm are shown. The UV chromatogram
for dA
60
/SPG was deconvoluted into two Gaussians to represent the complex
and free DNA, and each deconvoluted line is shown as a dotted line
(indicated by arrow). (B) Comparison of the RI and UV chromatograms for
S-dA
60
/SPG. The stoichiometric sample had no free S-dA
60
and the
complexation yield was almost 100%. The peak-top appeared at 17 min,
becoming
more
forward
than
dA
60
/SPG
(18 min)
and
close
to
tSPG
(16.2 min).