Biomedical Engineering Reference
In-Depth Information
d
n
4
y
3
n
g
|
3
Figure
4.13
Formation
of
arginine-grafted
bioreducible
polymer
(ABP)-coated
adenovirus complex.
cationic ABP polymer-coated Ad complex compared to naked Ad. In both
high and low coxsackievirus and adenovirus receptor (CAR)-expressing cells,
the ABP-coated Ad complex produced higher levels of transgene expression
and minimal toxicity than 25 kDa PEI. Moreover, the ABP-coated Ad
complex significantly reduced the innate immune response relative to naked
Ad. The combination of Ad with ABP polymer offers the potential to increase
the efficiency of vectors for gene therapy by shielding the virus from
deactivation by the immune system, and may make systemic administration
feasible.
Development of the transfection enhancement of liposomes with attributes
of high stability and easy handling in gene therapy is challenging. Li et al.
reported didodecyldimethylammonium bromide (DDAB, a cationic lipid)-
coated gold nanoparticles (DDAB-AuNPs), which enhanced the transfection
efficiency generated by two kinds of commercially available cationic
liposomes: Lipotap and DOTAP.
158
They showed that DDAB-AuNPs at the
optimal concentrations could produce higher gene expression and lower
cytotoxicity than lipoplex. The results indicated that DDAB-AuNPs increased
the cellular uptake efficiency of DNA molecules, which might account for the
enhancement of transfection efficiency.
Wang et al. recently developed the PLGA/folate-coated PEGylated
polymeric liposome core-shell nanoparticles (PLGA/FPL NPs) for co-delivery
of drug and gene.
159
Hydrophobic drugs could be incorporated into the core
and the cationic shell of the drug-loaded nanoparticles could be used to bind
