Biomedical Engineering Reference
In-Depth Information
The use of tissue engineering to deliver genes to stem cells has been impeded
by low transfection efficiency of the inserted gene and poor retention at the
target site. Recently, Jung et al. developed a sustained gene delivery system
using a fibrous 3D poly(
L
-lactide) scaffold coated with cell-permeable peptide/
DNA complexes (Figure 4.12).
152
The human-derived arginine-rich peptide
Hph-1 (YARVRRRGPRR) displayed a higher transfection efficiency and
lower toxicity in human adipose-derived stem cells (hADSCs) compared with
Lipofectamine. DNA/Hph-1 complexes were released from the scaffolds over
14 days and were successfully transfected into hASCs seeded on the scaffolds.
Target gene transfection for desired cell differentiation has recently become
a major issue in stem cell therapy. For the safe and stable delivery of genes into
hMSCs, Park et al. evaluated the differentiation capability of human
mesenchymal stem cells (hMSCs) using the SOX trio genes (master genes for
chondrogenic differentiation) as targets after modification with biodegradable
PLGA nanoparticles.
153
PEI was polyplexed with a combination of SOX trio
fused to reporter genes coated onto PLGA nanoparticles. They found that the
SOX trio complexed with PEI-modified PLGA nanoparticles led to increased
cell-uptake capacity and subsequent enhanced chondrogenesis of exogenous
gene-delivered hMSCs. Park et al. designed a nonviral gene delivery system
using nanoparticles, with emphasis placed on the ability of the system to
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Figure 4.12
Schematic diagram of a scaffold-supported sustained gene delivery
system.
