Biomedical Engineering Reference
In-Depth Information
(NPs)/DNA for effective and targeted gene delivery (Scheme 4.16A).
116
The
ternary complexes of PCL-g-PDMAEMA NPs/DNA/PGA-g-mPEG demon-
strated lower cytotoxicity and higher gene transfection efficiency than the binary
complexes in vitro. In vivo gene transfection experiments indicated that the ternary
complexes presented lower toxicity and higher protein expression in HeLa tumor-
bearing mice than the binary complexes.
Shen et al. have described a combined gene vector composed of PEI and a
nuclear protein (HMGB1) containing NLS (Scheme 4.16B).
117
The results of
cell viability studies suggested a lower cytotoxicity for the HMGB1/PEI
combined carriers. The ternary complexes were formed via electrostatic
interactions between DNA, HMGB1, and PEI. Transfection efficiencies of the
ternary complexes were higher than that of DNA/PEI complexes.
Wang et al. have developed a simple method to prepare PGA/PEI/DNA
ternary complexes to overcome the serum inhibitory effect of cationic polymers
(Scheme 4.16C).
118
Biocompatible PGA containing carboxyl groups could
self-assemble with positively charged PEI. The PEI/PGA combined carriers
showed lower cytotoxicity than 25 kDa PEI. The transfection efficiency of
these
d
n
4
y
3
n
g
|
3
terplexes
was
significantly
higher
than
that
of
25 kDa
PEI
or
Lipofectamine 2000 in 10% FBS-containing medium.
Scheme 4.16
Formation of self-assembled complexes: (A) PCL-g-PDMAEMA NPs/
DNA/PGA-g-mPEG;
(B)
HMGB1/DNA/PEI;
and
(C)
PGA/PEI/
DNA ternary polyplex.
