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Table 1. Physiological response measurements
Physiological Response Measurement
Unit of Measure
Observed Anatomical Entity
Frequency (Hz)
Amplitude
Electroencephalography (EEG)
Brain/Electrical
mL O 2 /min
Local Sp0 2
Functional Magnetic Resonance Imaging (fMRI)
Brain/Body
mL O 2 /min
Sp0 2
Functional Near Infrared Resonance Imaging (fNIR)
Brain/Body/Blood
Beats Per Minute
(BPM)
Pulse Waveform
Blood Volume Pulse
Cardiac Activity
Beats Per Minute
(BPM)
Sp0 2
Blood Tissue Oxygen Saturation & Pulse Oximetry
(Near Infrared Spectrophotometrics)
Cardiac Activity
Cardiopulmonary
Frequency (Hz)
Beats Per Minute
(BPM)
Auscultation Technique
Cardiac Activity
Beats Per Minute
(BPM)
Arterial Pulse
Cardiac Activity
Millimeters of mercury
(mmHg)
Arterial Blood Pressure
Cardiac Activity
Beats Per Minute
(BPM)
Amplitude
Frequency (Hz)
Electrocardiogram (ECG/EKG)
Cardiac/Electrical Activity
Variation in Heart Rate dur-
ing Respiration
Respiratory Sinus Arrhythmia (RSA)
Cardiopulmonary
Tidal Volume Estimation: Inductive Plethysmogra-
phy
Functional Residual Capac-
ity (FRC) Milliliters mL
Cardiopulmonary
Dimensional change in
Length
Strain Gauge Analysis
Pulmonary
Parts Per Million
(PPM)
Carbon Dioxide (CO 2 ) Concentration Analysis
Cardiopulmonary
Saccade
Dwell Time
Eye Movement Tracking
Eye/Movement
Skin/Gross Body Electrical
Conductivity-Resistance
Galvanic Skin Response (GSR)
Microsiemens Microhoms
Fahrenheit
Celsius
Temperature
Gross Body or Local
The reason for the validity of physiological re-
sponse and arousal as indicators of emotional state
and engagement is that there is an identified con-
nection between affective state (emotional state)
and the sympathetic nervous system. Hence, emo-
tion and engagement may trigger varying physi-
ological responses in different people. According
to McQuiggan, Lee and Lester (2006), “Because
physiological responses are directly triggered by
changes in affective state, biofeedback data such as
heart rate and galvanic skin response can be used
to infer affective state changes” (p. 60). However,
it is still unclear which details and aspects of the
emotional state are reflected by which particular
physiological responses, although there have been
advances in the temporal and spatial resolution
of physiological response measurement devices.
Regarding the current state of research concerning
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