Biology Reference
In-Depth Information
A
B
CA
1
Dopamine-
producing
cells
0.5
CC
Brain
0
200
m
m
Day 2
4th
0
1
2
5th instar
(Day)
C
D
1.5
1
1
0.5
0.5
0
0
0
0.01
0.1
1
10
100
Dopamine (
m
M)
E
2
BmDopR1
BmDopR2
1
0
4th
5th
Figure 3.2 Inhibition of JH biosynthesis by dopamine in the last (fifth) instar stadium in
Bombyx. (A) Dopamine-producing cells located in the medial portion of the day 0 fifth
instar Bombyx brain detected by a dopamine antiserum. (B) Stage-dependent inhibition
of JH synthesis by dopamine. CC
-
CA complexes from larvae of various stages were
cultured with or without 100
m
M dopamine for 6 h followed by the determination of
JH synthetic activity (N¼3
-
5, SE). Ratio represents the quantity of the amount of JH syn-
thesized by the CA cultured with dopamine divided by the amount without dopamine.
Note that the CA from only fifth instar larvae were suppressed to synthesize JH.
