Biology Reference
In-Depth Information
Figure 11.2 Adult stem cells originate from the larval epithelium in X. laevis intestines
cultured in vitro. (A) Schematic diagram of tissue recombination and organ culture.
Tubular fragments were isolated from the small intestine just behind the bile duct junc-
tion (bd) of stage 57-wild-type (Wt) or transgenic (Tg) tadpoles that express GFP ubiq-
uitously under the CMV promoter. They were slit open lengthwise and separated into
epithelial (E) or nonepithelial tissues (non-E). Each epithelium was then recombined
with homologous or heterologous non-E. The four kinds of recombinant intestines were
placed on membrane filters on grids and cultured in the medium containing thyroid
hormone.
*
Type of E/type of non-E. (B) Recombinant intestines cultured for 5 (a
-
d)
and 7 days (e
-
h). Sections were double-stained with anti-Shh (red) and anti-GFP (green)
(a
-
d), or anti-IFABP (red) and anti-GFP (green) antibodies (e
-
h). Adult progenitor/stem
cells positive for Shh (arrowheads) express GFP in Tg/Tg (a) and Tg/Wt intestines (b), but
do not in Wt/Tg (c) and Wt/Wt intestines (d). Similarly, adult differentiated cells positive
for IFABP (arrowheads) express GFP in Tg/Tg (e) and Tg/Wt intestines (f), but do not in
Wt/Tg (g) and Wt/Wt intestines (h). Bars: 10
m.
m
