Biology Reference
In-Depth Information
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Figure 6.5 Expression of pitx2 in the lateral plate mesoderm (LPM). The embryos at mid-
somitogenesis (17
18 somite stage) were analyzed with pitx2 antisense RNA probe. (A)
Control F3 embryo. (B
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F) rev F3 embryos. (G, H) Transverse section of rev embryos.
Among these embryos, 43% exhibits pitx2 expression in the left LPM (B) as in normal
embryos (A). In another 43% of the embryos from rev, pitx2 was expressed bilaterally
without apparent L/R bias (C). In 4% and 6%, pitx2 expression was observed bilaterally
but in left- and right-biased fashions, respectively (D, E). Four percent exhibited pitx2
expression only on the right side (F). Sections in (G, H) reveal that pitx2 expression
domain locates in the left LPM. Right-sided expression of pitx2 is also detected in the
LPM as shown in (H). Dashed lines in (B) and (C) denote regions of cross-section depicted
in (G) and (H), respectively. Left LPM (open arrowhead), right LPM (closed arrowhead).
(A-F) Dorsal view, anterior to the top. Bilaterally symmetrical pitx2 signal in the anterior
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