Biology Reference
In-Depth Information
CHAPTER THREE
The Role of MicroRNAs in
Muscle Development
Nicholas S. Sokol
Contents
1. Introduction
60
60
2. Muscle miRNA Regulatory Networks
60
2.1. Identification of muscle miRNAs
2.2. Genomic organization of muscle miRNAs
61
64
2.3. Transcriptional control of muscle miRNAs
2.4. Posttranscriptional regulation of muscle miRNAs
64
65
2.5. mRNA targets of muscle miRNAs
3. Functional Analysis of miRNAs During Muscle Development
65
66
3.1. Muscle phenotypes of zebrafish and mouse
dicer
mutants
3.2. Muscle phenotypes of miRNA mutants
68
73
4. miRNAs in Muscle Stem Cells
5. Concluding Remarks
74
References
75
Abstract
MicroRNAs play essential roles during animal development, including in devel-
oping muscle. Many microRNAs are expressed during muscle development and
some, like
miR-1
and
miR-133
, are muscle specific. Muscle microRNAs are
integrated into myogenic regulatory networks: their expression is under the
transcriptional and posttranscriptional control of myogenic factors, and they in
turn have widespread control of muscle gene expression. This review sum-
marizes recent work characterizing the function of microRNAs in muscle biology
and specifically focuses on the genetic analysis of muscle microRNAs in a
variety of model organisms including worms, flies, zebrafish, and mice.
Department of Biology, Indiana University, Bloomington, Indiana, USA
