Assessment of Drug-Induced Cardiotoxicity in Zebrafish - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 49

Figure 4.1 Drug effects on zebrafish heart rate and AV ratio. Zebrafish (2dpf) were incubated at 28 C
with selected drugs at various concentrations for 4 h (a and c). The bar graph for each drug shows associated
drug-induced changes in atrial contraction rates. The ventricle is more sensitive to the presence of
cardiotoxic compounds and usually showed amore significant decrease in activity than the atrium. (b and d).
Normal AV contraction ratios of 1:1 increased to 10:1 or higher with increasing drug concentration. A
notable exception was clomipramine, which maintained a 1:1 AV ratio with increasing drug concentration
despite a decrease in heart rate. ANOVA with a post-hoc Dunnett's test (based on raw data) was used to
identify concentrations that significantly altered heart rate, P <

0.05 was considered significant, and error

bars are equal to 1 SD.