Biomedical Engineering Reference
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regeneration (1 dpa) are conserved between nonamputated control and regenerating fin
tissue in the adult and larval fin regeneration models (Mathew et al., 2009). Taken
together, these data suggest that there is a high probability of common hierarchical
molecular signaling pathways across larval and adult regeneration systems.
22.8 SUMMARY
Regeneration is a research area that has captivated the imagination of biologists for
decades, yet there are currently numerous gaps in our understanding of the complex
pathways and interactions that dictate regeneration. The methods described here will
facilitate the molecular dissection of the signaling events that control wound healing,
blastema formation, regenerative outgrowth, and termination of regenerative signal-
ing. Importantly, studies designed to probe the signaling events that choreograph
regeneration will provide new avenues for comparative studies in mammalian models
in the hope of developing novel therapeutics to help slow and/or prevent tissue loss
from injury, aging, and disease, thereby improving the quality and duration of life.
ACKNOWLEDGMENTS
We thank Jill Franzosa and Margaret Corvi for helpful discussions and the critical
review of this manuscript. This research was supported by the NIEHS Environmental
Health Sciences Center Grant No. ES00210 and NSF Grant No. 0641409.
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