Assessment of Effects on Visual Function in Larval Zebrafish - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 194

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Figure 15.5 Assessment of visual function and locomotor activity of zebrafish larvae at 8dpf after 5 days
of treatment withbisoprolol in: (a) OMR assay: Themean and standard errors are shown for 6 runs. Each run
used a group of 10 larvaetreatedwith compound or DMSO control. P < 0.005 in a Student's t test comparing
control with treated groups. Treatment with 300 mM bisoprolol significantly inhibited OMR; (b) OKR
assay: Data represents the mean andstandard error of groups of 12 larvae assessed for the number of
saccades in 1 min in the & clockwise and & counterclockwise directions. No group was significantly
different from control in one-way ANOVA and Dunnett's multiple comparison tests; (c) Locomotor
activity: Data represents the mean and standard error of groups of 10 larvae assessed by livevideo-tracking
of movement in 96-well plates for 45 minutes. P < 0.01 in one-way ANOVA and Dunnett's multiple
comparison tests. Treatment with 30-300 mM bisoprolol significantly inhibited locomotor activity.
(Reprinted from Richards et al. (2008) with permission from Elsevier)