Zebrafish: A Predictive Model for Assessing Seizure Liability - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 166

13.3.2 Concentration Effect on Peak D

Using data shown in Fig. 13.2, we identified the longest D traveled (peak D )for
each compound concentration (Table 13.2). Since the negative control compound,

Table 13.2 Concentration Effects on Peak D and Time Point at Peak D

Time point at
peak D (min) Compound Concentration (mM) Peak D (mm)

PTZ 1.25 66 30

2.5 245 21

5 226 7

4-AP 0.05 50 4

0.15 63 9

0.3 266 7

Picrotoxin 0.1 73 37

0.5 131 15

1 116 10

Strychnine hemisulfate 0.05 18 9

0.1 13 7

0.5 33 2

Methoxychlor 0.001 N.O. N.O.

0.005 N.O. N.O.

0.025 65 5

Amoxapine 0.028 N.O. N.O.

0.056 39 60

0.084 67 41

Aminophylline hydrate 1 N.O. N.O.

5 39 19

10 21 11

Bicuculline methiodide 0.8 20 34

2.5 31 55

5 25 53

Enoxacin 0.5 16 36

2 16 35

5 15 11

Lidocaine 0.25 N.O. N.O.

1 N.O. N.O.

5 N.O. N.O.

N.O., no peak observed.