Zebrafish: A Predictive Model for Assessing Seizure Liability - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 163

Table 13.1 Compounds Used to Validate Zebrafish Seizure Assay

Compound Mechanism of action Concentrations (mM)

PTZ GABA antagonist 1.25, 2.5, 5

4-AP Norepinephrine reuptake

inhibitor

0.05, 0.15, 0.3

Picrotoxin Blocks potassium channel 0.01, 0.05, 0.1, 0.5, 1

Strychnine hemisulfate Synthetic organochlorine 0.001, 0.005, 0.02. 0.05, 0.1, 0.5

Methoxychlor Adenosine antagonist 0.001, 0.005, 0.025

Amoxapine Glycine receptor antagonist 0.028, 0.056, 0.084

Aminophylline hydrate GABA antagonist 1, 5, 10

Bicuculline methiodide GABA antagonist 0.8, 2.5, 5

Enoxacin GABA antagonist 0.1, 0.5, 1, 2, 5

Lidocaine Blocks sodium channel 0.25, 1, 5

previous studies. If no effects were observed in initial tests, additional lower or higher
concentrations were assessed. Compound concentrations are shown in Table 13.1.

13.2.4 Assessment of Drug Effects on Distance
Traveled

The VideoTrack System separated movement of single zebrafish in each well into
three speeds: low (less than 4mm/s), medium (between 4 and 20 mm/s), and high
(greater than 20 mm/s), and movements were color coded (Fig. 13.1). Black lines
represent low-speedmovement. Green lines (and green areas) indicate medium-speed
movement. Red lines (and red areas) indicate high-speed movement. PTZ exposure
induced high-speed movement (left panel, red areas).

Figure 13.1 Graphical representation of movement of single larval zebrafish (left, DMSO control; right,
5mM PTZ) during 60min. Recording commenced immediately after treatment with PTZ. Black lines
represent low-speed movement (less than 4mm/s). Green lines (areas) indicate medium-speed movement
(between 4 and 20mm/s). Red lines (areas) indicate high-speed movement (greater than 20mm/s). PTZ
exposure induced high-speedmovement (right). (See the color version of this figure in Color Plates section.)