Zebrafish: A Predictive Model for Assessing Cancer Drug-Induced Organ Toxicity - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 140

Figure 11.4 Toxicity in zebrafish heart, CNS, liver, and kidney 48 h after compound removal. Heart and
CNS (left panels): 48 h after removing 17-DMAG and HDAC inhibitor X, pericardial edema (black arrow)
was still visible. No toxicity in heart or CNS was observed 48 h after removing paclitaxel and SMA-838.
Liver and kidney (right panels): 48 h after removing 17-DMAG, zebrafish exhibited discolored liver (yellow
outlines) and trunk edema (red arrows); 48 h after removing HDAC inhibitor X, liver discoloration was still
present, and trunk edema was observed; 48 h after removing paclitaxel, zebrafish appeared normal; 48 h
after removing SMA-8383, 40%of animals exhibited discolored liver (yellow outline). Scale bar ¼0.5mm.
(See the color version of this figure in Color Plates section.)

effects were irreversible. After 24 h treatment, cell death was observed in CNS and
liver, but not in heart or kidney.

11.3.4.5 Zebularine Based on the absence of toxicity observed during lethality

assessment and limited compound availability, organ toxicity and cell death were not
evaluated separately.