Biomedical Engineering Reference
In-Depth Information
(a)
(b)
1600
1200
**
**
**
**
1200
800
**
*
*
*
800
***
***
400
400
0
0
50
μ
m Dex 0.1% DMSO
0.01
m
Azamulin concentration
μ
m
0.1
μ
1
μ
m
10
μ
m
100
μ
m
50
μ
m Dex 0.1% DMSO
0.01
μ
m 0.1
μ
m
Rifampicin concentration
1
μ
m
10
μ
m
100
μ
m
Figure 9.1
CYP3A4 inhibition and induction using microplate-based whole zebrafish CYP3A4 assay. Two dpf zebrafish were treated with azamulin or rifampicin
for 24 h and zebrafish CYP3A4 activity was measured using a CYP3A4 chemiluminogenic substrate. Zebrafish treated with 50
m
M dexamethasone (Dex) were
SD.
p
0.05,
p
0.01, and
p
used as a positive control. Data are expressed as mean
0.001 compared with 0.1% DMSO control. RLU
¼
relative luminescence
<
<
<
units.
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