Biomedical Engineering Reference
In-Depth Information
Fig. 14.5
Primer-induced autonomous synthesis of DNAzyme units on template DNA by using
polymerase/dNTPs and a nicking enzyme as the biocatalyst (Reproduced from Ref. [
28
]by
permission of John Wiley & Sons Ltd.)
a new site for the initiation of replication. Thus, the polymerase completes the
replication of the DNAzyme, and the reactivated replication at the scission site dis-
places the already synthesized DNAzyme. Subsequently, in the presence of hemin,
the autonomous synthesis of the G-quadruplex DNAzyme structure is activated.
The DNAzyme catalyzes the oxidation of ABTS
2
by H
2
O
2
or stimulates the
generation of chemiluminescence in the presence of luminol/H
2
O
2
. The proposed
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