DNA Walking Devices - DNA Nanotechnology: From Structure to Function - page 263

Fig. 12.8 Overview of the DNAsome system (Reprinted with permission from Macmillan
Publishers Ltd: Ref. [ 29 ]. Copyright 2010 Nature Publishing Group)

external intervention [ 29 ]. Actually, the design of this walking system followed the
enzyme-driving mode with a difference of the organic groups carried by the walker
(Fig. 12.8 ). The mechanism of this system could be generalized into several steps:
DNA-templated amine acylation, DNAzyme-catalyzed cleavage, and dissociation
of the 5 0 fragment of the expended substrate, which resulted in the walker resting
at the final station on the template covalently linked to the final multistep reaction
product. This system, which mimicked the biosynthesis under mild conditions in
cell, could help to improve the ease, speed, and efficiency of multistep synthesis.

12.5 Summary and Future Directions

In general, DNA walker could be included into two points: (1) the systems were
composed of a track and a walker which could associate and dissociate according
to the design; (2) the power of DNA walker was derived from DNA hybridization,