Biomedical Engineering Reference
In-Depth Information
In another strategy, Yan and Yu et al. showed that gold nanoparticle arrays
fabricated by EBL method can also be used as nodes for building DNA origami
networks, rather than simple absorption position [
72
]. Fixed-length DNA origami
nanotubes, modified with multiple thiol groups near both ends, were used to connect
surface patterned gold islands. Later, a similar work was done by Davis' group,
but the interactions between DNA origami and gold nanoparticle nodes were DNA
hybridizations through sticky ends base pairing, instead of thiol groups [
73
].
10.7
Perspectives
In summary, we have witnessed explosive growth of research interest in DNA
origami technique since its invention in 2006. Compared with Rothemund's original
work, now people are able to build complex 2D and 3D artificial nanostructures
with defined geometry; look into its inherent physical, mechanical, electrical, and
biological properties; assemble higher-order and larger patterns by using individual
origami as building block; and narrow the gap between top-down and bottom-up.
Applications of DNA origami, although not included in this chapter, are also a fast-
growing research field. However, looking towards the future, the current research
on DNA origami is still in its early stages, and more challenges remain to be
solved. Fundamentally, we are still not clear on the formation mechanism of DNA
origami and wonder what on earth could improve the yield, quality, and stability;
simplify the preparation; and make it cheaper. From the structural point of view,
it is constantly expected to build more complex, larger, and stronger structures
with controlled addressability and flexibility that could be manipulated by present
scientific instruments. Finally, it is also expected that more properties could be
elucidated to help the combination of DNA origami with other techniques to find
valuable applications.
References
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3. Seeman NC (2000) DNA nicks and nodes and nanotechnology. Nano Lett 1(1):22-26
4. Winfree E, Liu F, Wenzler LA, Seeman NC (1998) Design and self-assembly of two-
dimensional DNA crystals. Nature 394(6693):539-544
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(2009) From molecular to macroscopic via the rational design of a self-assembled 3D DNA
crystal. Nature 461(7260):74-77
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