DNA Origami Nanostructures - DNA Nanotechnology: From Structure to Function - page 206

Fig. 10.3 Efforts towards new assembly strategies, improving purification, and studying prop-
erties. ( a ) Isothermal assembly (Reprinted with the permission from Ref. [ 25 ]. Copyright 2008
American Chemical Society). ( b ) Using dsDNA scaffolds (Reprinted with the permission from
Ref. [ 26 ]. Copyright 2009 American Chemical Society). ( c ) Improving the recovery yield through
electroelution in agarose gel (Reprinted with permission from Macmillan Publishers Ltd: Ref. [ 29 ],
copyright 2011). ( d ) Melting behavior revealed with in situ thermally controlled AFM (Reprinted
with the permission from Ref. [ 36 ]. Copyright 2012 American Chemical Society). ( e ) Mechanical
study using magnetic tweezers (Reprinted with the permission from Ref. [ 38 ]. Copyright 2011
American Chemical Society)
Towards improving the yield and shorting the annealing time used in Shih's
multilayer DNA origami strategy, his group recently made two new improvements
[ 28 ]. In the first method, they made underwound double helices in the structure
that were stabilized by the binding of intercalator dyes. This enhanced affinity of
intercalators can be exploited for the design of functionalities that are targeted
to the underwound helices of the nanostructure via multivalent intercalation. In
the second method, they explored the role of staple-break selection and found