Biomedical Engineering Reference
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Fig. 2.2
(
a
) A long single-strand DNA generated by RCA with repeated aptamer units to
recognize target proteins (Reproduced from Ref. [
67
], by permission of John Wiley & Sons Ltd.).
(
b
) TX tiles with thrombin aptamers assembled 1D periodic protein nanowires with AFM imaging
(Reproduced from Ref. [
68
], by permission of John Wiley & Sons Ltd.). (
c
) 2D array formed
by four types of DX tiles utilized with PDGF aptamer and thrombin aptamer in separate tile to
assemble multiplexed protein nanoarrays. Two adjacent proteins are demonstrated to be 32 nm by
AFM imaging and height analysis (Reprinted with the permission from Ref. [
69
]. Copyright 2007
American Chemical Society). (
d
) S-shaped patterned thrombin proteins on rectangular-shaped
DNA origami (Reprinted with the permission from Ref. [
69
]. Copyright 2007 American Chemical
Society). (
e
) Supramolecular linear nanowire formed by the binding of aptamers to thrombin by
linear assembling unit and Y-shaped nanowire by a combination of triangle and linear assembling
units (Reproduced from Ref. [
71
] by permission of The Royal Society of Chemistry)
protein assembly can be generated. The robust protein-aptamer binding with
efficient RCA reaction allows the creation of complex linear nanostructures with
DNA and protein as the building blocks.
In order to make the 1D patterning more rigid, Yan's group [
68
] reported the
use of triple-crossover (TX) DNA tiles with thrombin aptamers protruding from
the tile plane at the end of the stem. The tiles were subsequently assembled into
1D periodic nanowires with thrombin aptamers dangling outwards to recognize
thrombin proteins, as shown in Fig.
2.2
b. The assembly structure was clearly
characterized by atomic force microscopy (AFM) and the height analysis. The
position and rotational orientations of proteins can be defined by adjusting the length
of the stem. Meanwhile, the distance between proteins on the assembly can be
tuned through the alternation of the number of different tiles. The precise location
of protein programmed by aptamers on DNA nanostructures proves it as a novel
platform for building the nanoarchitecture of protein.
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