Biomedical Engineering Reference
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Fig. 8.14
Fish sperm DNA-assisted synthesis and valence separation of highly stable silver
nanoparticles bearing a discrete number of DNA ligands (Reproduced from Ref. [
67
] by permission
of The Royal Society of Chemistry)
We recently realized a facile strategy capable of synthesizing highly stable
2 nm silver nanoparticles that were well-suited for a gel electrophoretic isolation
of their discrete DNA conjugates (Fig.
8.14
)[
67
]. Our method was based on the
chemical reduction of Ag
C
in the presence of shortened fish sperm DNA (FSDNA).
The as-synthesized AgNPs could be quickly concentrated by butanol extraction
(to remove aqueous phase), and the concentrated AgNPs appeared as a clean and
sharp band in an agarose gel. As another merit, the FSDNA-templated AgNPs
exhibited an excellent stability at extremely high salt concentrations (3.0 M Na
C
or
0.3 M Mg
2C
), which is highly desired for the stabilization of a DNA nanostructure
that directs the nanoparticle assembly.
We then modified the AgNPs with a doubly thiolated DNA complex in order to
maintain a strong bonding of DNA on the 2 nm AgNPs. Note that the two thiols were
appended on a blunt end of the double-stranded DNA ligand, one at 5
0
and the other
at 3
0
position, while the other end of the DNA ligand was a single-stranded overhang
for assembly purposes. The AgNP-DNA conjugates bearing a discrete number of
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