Biomedical Engineering Reference
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Fig. 7.12 Components ( a ), assembly ( b ), and operation ( c ) of a paper-based device that performs
recombinase polymerase amplification ( RPA ). ( a ) Devices are composed of five components: a
base layer made of acetate and double-sided adhesive covered by a protective layer of paper; a
second acetate layer to aid alignment of other components; a sample wick strip patterned with
melted wax for absorbing exactly 10
L of sample; a cellulose pad for holding a master mix
solution; and a glass fiber pad for holding magnesium acetate solution. ( b ) Devices are assembled
by peeling off the protective paper layer and assembling each device component in the order shown.
The white pellet shown in the first step is composed of lyophilized enzymes and reagents. ( c )The
user operates the device by pipetting reagents on the appropriate pads, dipping the wick into the
sample, and mixing reaction components by folding the device in half (Reproduced from Ref. [ 71 ]
with permission of The Royal Society of Chemistry) (Color figure online)
fancy devices may not have enough competitive edge compared with the simplest
Eppendorf tubes in real applications because of their complexity or cost. They
normally require fine operations, hence well-trained individuals. How to deal with
DNA analysis under small scale in an as simple way as possible is a new research
focus and will be growing fast.
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