Biomedical Engineering Reference
In-Depth Information
Chapter 7
Microfluidic Tools for DNA Analysis
Yi Zhang and Xingyu Jiang
Abstract This chapter introduces the use of microfluidic tools for DNA analysis. It
will cover both qualitative analysis and quantitative analysis. A microfluidic device
typically implies multicomponent integration. Most reviews in scientific journals
discuss microfluidics by a sequential introduction for each component. In order to
present the great power of microfluidics as emerging tools for DNA research, we
organized each section according to the primary function that can be achieved by
a kind of microfluidic device, and emphasize the primary innovation leading to
the unique function for each specific device. As microfluidic tools showed many
distinct advantages over existing approaches and thus hold dramatic commercial
potential, we will also discuss the problems during the commercialization process
of microfluidic devices. DNA analysis we discussed herein includes amplification,
detection, sequencing, counting, sizing, and weighing, and our perspective covers
a wide range of related fields including chemistry, molecular biology, physics,
and micro/nano-fabrication technologies, which reveals that DNA analysis on
microfluidic devices is a highly interdisciplinary subject and will have lasting impact
among biologists, chemists, physicians, and engineers.
Keywords Microfluidic chip • DNA • Miniaturization • Amplification • Solid
phase
Sample-in-answer-out
Droplet
Structural
variation
Cantilever
• Qualitative analysis • Quantitative analysis
Y. Z h a n g
Department of Biomedical Engineering, College of Engineering, Peking University,
Beijing 100871, China
National Center for Nanoscience and Technology, Beijing 100190, China
X. Jiang ( )
National Center for Nanoscience and Technology, Beijing 100190, China
e-mail: xingyujiang@nanoctr.cn
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