Enzymes as Biocatalysts for Lipid-based Bioproducts Processing - Food and Industrial Bioproducts and Bioprocessing - page 343

Substrate

Selection of
enzyme

Production
of enzyme

Application
of enzyme

Downstream
process

Enzymatic
production

Improvement
of enzyme

Characterization
of enzyme

Product

Enzyme-reaction
identification;
High-throughput
screening; Functi-
onal genomics;
Extremophiles

Immobilization;
Enzyme reuse;
Reaction protocol
design,modeling
& optimization

Directed evolution;
Microbial
genomics;
Proteomics;
Bioinformatics

Purification;
Kinetics;
DNA sequence-
enzyme structure
modeling

Economic
estimation;
Reactor design;
Integration;
Patenting;

Gene expression;
Fermentation;
Purification &
formulation

Product recovery;
Solvent handling

Figure 14.6 Typical flowchart of engineering enzyme as catalyst for efficient bioprocessing.