Biomedical Engineering Reference
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O
R
NH
HO
O
O
O
OH
O
O
O
or
O
RCOOH
O
R
R
O
R
Cl
R
O
NH
2
NH
HO
O
O
HO
O
O
O
O
O
OH
OH
RCOOH
carbodimine (NHS, EDC.)
O
R
NH
HO
O
O
O
OH
Figure 2.12
Synthesis of acyl-chitosans.
The inhibitory rates of DOX-OCH nanoparticle suspension to different human cancer cells
(A549, Bel-7402, HeLa, and SGC-7901) significantly outperformed that of DOX solution.
2.4.1.3 Acyl Chitosan Derivatives from Coupling Reactions
An aniline pentamer chitosan derivative with electroactivity was prepared by a coupling
reaction [38]. Due to its amphiphilic property, this derivative is able to self-assemble into
spherical micelles, which makes the potential application of these polymers in drug deliv-
ery possible. The use of these polymers as scaffold materials in neuronal tissue engineer-
ing was evaluated; noncytotoxicity, degradability in the presence of enzymes, and
biocompatibility were observed. Moreover, differentiation of PC-12 cells seeded on pure
chitosan and on the three electroactive samples with 2.5%, 4.9%, and 9.5% AP, respectively,
in the presence of exogenous nerve growth factor (NGF) was assessed for up to 5 days. The
PC-12 cells on samples containing AP showed neurite extension, and some of them even
formed intricate networks, while those on chitosan showed much fewer neurites.
Linoleic acid has been covalently conjugated to chitosan or chitosan derivatives via a
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-mediated (EDC-mediated) reaction to
generate amphiphilic chitosan derivatives. Linoleic-chitosan nanoparticles have been used
for the adsorption of trypsine (TR) [40]. Environmental factors (e.g., pH, concentration of
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