Biomedical Engineering Reference
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Figure 9.25
(a) SEM image depicting surface morphology of the
N
-succinyl-chitosan/aldehyde HA composite hydrogel
encapsulated with chondrocytes after 24 h culture. (b) SEM image depicting the morphology of encapsu-
lated chondrocytes after 24 h culture. (From Huaping Tan, H. P. et al. 2009.
Biomaterials
30: 2499-2506. With
permission.)
optimum results were obtained with chitosan hydrogel fragments of 55-70% DD and
1.5% chitosan.
9.5.3.2 Chitosan-Based Porous Hydrogels-Chondrocytes Construct
Chondrogenesis has been described on chitosan scaffolds. Since cartilage is avascular, car-
tilage nutrition, and metabolism are mainly influenced by local diffusion and cell-matrix
Figure 9.26
Histological morphology of constructs prepared with human articular chondrocytes (63-year-old female patient)
and chitosan hydrogel (copolymer = 1.5%, DD of chitosan = 59.6%) and cultured for 20 days (a) or 63 days (b, c).
Area: ≤50 μm
2
“↓”, or >100 μm
2
“H”. (From Montembault, A. et al. 2006.
Biochimie
88: 551-564. With permission.)
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