Application of Chitosan-Based Biomaterials in Tissue Engineering - Chitosan Based Hydrogels: Functions and Applications - page 417

Isolated cells Gel precursors Gel cross-linking

Tissue generation &
scaffold degradation

Degradation &
ECM synthesis

Figure 9.14
Cell encapsulation strategies involve mixing cells with precursors in a liquid solution followed by gelation and
encapsulation of cells. (From Nicodemus, G. D. and Bryant, S. J. 2008. Tissue Eng Part B 14: 149-165. With
permission.)

Incubate at 37°C

Add chitosan-based precursor solution
and first-layer gel formation via environment stimuli

Seed second layer of the cells

Attachment

Add chitosan-based precursor solution
and second-layer gel formation via environment stimuli

n th layer of the cell

n th layer of gel

Second layer of gel
First layer of gel

Second layer of the cell
First layer of the cell

Figure 9.15
LBL encapsulation of cells.