Enzyme Immobilization on Chitosan-Based Supports - Chitosan Based Hydrogels: Functions and Applications - page 392

F. Chitosan-tethered PANMV membrane (d-EDC and NHS) II-iii (GA) [85]

E. Urea sensor ZnO-chitosan nanobiocomposite film (a) I-i [113]

Urease and
glutamate
dehydrogenase

E. Urea sensor Fe 3 O 4 -chitosan precipitations (a) I-i [101]

Yeast alcohol
dehydrogenase

F. Calcium phosphate-mineralized chitosan-alginate

microcapsule (i)

III-i [153]

Notes: Application of immobilization enzyme : (A) food industry; (B) pharmaceutical industries; (C) environmental industries; (D) other industries; (E) biosensors; (F)
separation, purification, and recovery of enzymes; (G) immobilization study.
Support preparation methods : (a) solvent evaporation method; (b) phase-inversion technique; (c) ionotropic gelation method; (d) chemical cross-linking method; (e)
freezing-thawing treatment; (f) electrodeposition method; (g) electrospinning method; (h) chemical modified; (i) PECs; (j) LBL self-assembly.
Immobilizations methods : (I) Adsorption of enzyme on support: (I-i) physical adsorption, (I-ii) affinity by metal ion, (I-iii) affinity by dye, (I-iv) affinity by ChBD; (II)
chemical cross-link by covalent binding: (II-i) in the presence of enzyme solution, (II-ii) in the absence of enzyme solution (enzyme may be absorbed or entrapped),
(II-iii) on agents-activated support, (II-iv) cross-linking by covalent binding of enzyme to one agent-activated support following cross-linking with the other agent;
(III) entrapment: (III-i) enzyme within a polymer network, (III-ii) covering a film on the surface of enzyme; (IV) electrochemically conjugate protein; (V) Enzyme-
chitosan conjugate.
Abbreviations :
Enzyme : α-l-arabinofuranosidase-Ara; Chitin-binding domain—ChBD; β-d-glucopyranosidase—βG; α-l-rhamnopyranosidase—Rha; carcinoembryonic anti-
gen—CEA; A 20-mer single-stranded oligodeoxyribonucleotide—ssODN.
Application : Galactooligosaccharides—GOS.
Agents : α-cyano-4-hydroxycinnamated—ACHCA; (3-aoryloxypropyl) dimethoxy methyl silane—APDMOS; 3-amino propyl tri ethoxy silane—APTES; 18-crown-6
ether—CE; 2-diethylaminochloroethane—DE; 1,2-7,8 diepoxyoctane—DEP; epichlorohydrin—ECO; 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochlo-
ride—EDC; glutaraldehyde—GA; glycidol—Gly; γ-glycidoxypropyltrimethoxysilane—GPTMS; N , N -methylene-bis-(acrylamide)—MBA; methyltrimethoxysi-
lane—MTOS; N -hydroxysulfosuccinimide—NHS; poly(thiophene-3-acetic acid)—PTAA; dimethylsuberimidate dihydrochloride—SUB; tetraethoxysilane—TEOS;
tetrakis(2-hydroxy ethyl) orthosilicates—THEOS; tetramethoxysilane—TMOS; sodium tripolyphosphate—TPP; tetrasodium pyrophosphate—TSPP.
Supports : Acetyl Yellow 9—AY9; cibacron blue F3GA—CB; carboxymethylpullulan—CMP; carbon nanotube—CNT; ferrocene monocarboxylic acid functionalized
Fe 3 O 4 -SiO 2 nanoparticles conjugate—FMC-AFSNPs; layer-by-layer—LBL; glassy carbon electrode—GCE; Montmorillonite—MMT; 3-mercaptopropionic acid—
MPA; multiwalled carbon nanotube—MWCNT; nanostructure gold—NG; Poly(vinyl alcohol)—PVA; polyelectrolyte complexes—PECs; polystyrene sulfonate—
PSS; single-walled carbon nanotube—SWCNT.