Biomedical Engineering Reference
In-Depth Information
TAble 8.2
Enzymes Immobilized on Chitin/Chitosan-Based Materials
Immobilization Method
(Cross-Linking Agents)
Enzyme
Application
Support (Preparation Method—Cross-Linking Agents)
References
α-Amylase
B.
Reversed chitosan-alginate beads (i)
III-i
[91]
F.
Chitosan microbeads (a and d-GA)
II-i
[26]
F.
Chitosan-MMT composite beads (b and d-GA )
II-iii (GA)
[145]
F.
Chitosan-MMT composite beads (b and d-GA )
II-iii (GA)
[145]
β-Amylase
Acetylcholinesterase
E. Pesticide determination
Chitosan-tethered PANMV membrane (d-EDC and NHS)
II-iii (GA)
[86]
E.
Nano-CaCO
3
-chitosan composite film (f )
I-i
[151]
Acid phosphatase
F.
Chitosan beads (b and d-GA)
II-iii
[29]
F.
Chitosan-ZrO
2
composite beads (b and d-GA)
II-iii
[115]
Acylase
D. Biofouling control in the
membrane bioreactor
Magnetic MIEX-PSS-chitosan microspheres (j)
I-i or II-iii (GA)
[109]
Alliinase
F.
Succinyl anhydride-chitosan as pH-sensitive support (h)
II-iii (GA)
[71]
l-Asparaginase
B.
Carboxymethyl konjac glucomannan/chitosan nanocapsule (i)
III-i
[95]
Catalase
A. Degradation of H
2
O
2
Chitosan beads (d-diphosphate and glyoxal and GA)
II-iii (GA)
[80]
F.
Cu
2+
-chitosan-beads (d-GA)
I-iii and II-iii
[50]
F.
CB-chitosan beads (d-diphosphate and glyoxal and GA)
I-iii and II-iii
[51]
F.
Poly(itaconic acid) grafted and/or Fe
3+
ions incorporated
chitosan membranes (b and h)
I-i or I-ii
[76]
Anti-CEA
E. Immunosensor
Anti-CEA/nano-Au-chitosan/NG/GCE films (j)
I-i
[124]
d-Carbamoylase
B. d-Amino acids production
Alginate-chitosan capsules (i)
III-i
[90]
Cellulase
F.
Polyanionic modified PVA-coated chitosan beads (i)
II-iii (ECO)
[100]
Cholesterol esterase
E. Total cholesterol sensor
Silica-chitosan-MWCNT hybrid composite film (d-MTOS)
II-iii (GA)
[144]
Cholesterol oxidase
E. Total cholesterol sensor
Silica-chitosan-MWCNT hybrid composite film (d-MTOS)
II-iii (GA)
[144]
E. Cholesterol biosensor
Silica-chitosan-MWCNT hybrid composite film (d-MTOS)
III-i
[143]
E. Cholesterol biosensor
Chitosan-alginic acid complexation network (i)
III-i
[92]
E. Cholesterol biosensor
CeO
2
-chitosan hybrid composites (a)
I-i
[118]
Chymotrypsin
F.
Chitosan -biopolymer complexation beads (b)
II-iii (GA or Gly or ECO)
[23]
DNA
E.
Chitosan films (f)
II-iii (GA)
[60]
DNA-Cu(II)
E. Measurement of H
2
O
2
DNA-Cu(II)-chitosan complexation films (i)
III-i
[98]
Anti-factor B
E. Detection of factor B
Factor B-alginate conjugate/Chitosan films (i)
V (EDC)
[93]
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