Optical Imaging - Correction Techniques in Emission Tomography - page 269

FIGURE2.7: Imaging-guided gene therapy paradigm of experimental
gliomas. Protocol for identification of viable target tissue and assessment of
vector-mediated gene expressioninvivoin a mouse model with three subcu-
taneous growing gliomas. Row 1: localization of tumors by MRI. Row 2: the
viable target tissue as depicted by [ 18 F]FDG-PET. Note the signs of necro-
sis in the lateral portion of the left-sided tumor (arrow). Rows 3{4: following
vector-application into the medial viable portion of the tumor (arrow) the
\tissue-dose" of vector-mediated gene expression is quantied by [ 18 F]FHBG-
PET. Row 3 shows an image acquired early after tracer injection, which is
used for coregistration. Row 4 displays a late image with specific tracer accu-
mulation in the tumor that is used for quantification.