Biomedical Engineering Reference
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FIGURE2.2: Longitudinal BLI analysis of the growth of subcutaneous brain
tumors. CD1 nu/nu mice were injected twice with 1*10
6
human U87EGFR
glioma cells that stably express rey luciferase. At different days post tumor
implantation the tumor volume was measured with the help of a caliper (A);
tumor volume = 0.52
length
width
2
). Furthermore, tumor activity was
assessed by BLI measurements 10 min after intraperitoneal injection of 2 mg
D-luciferin using the IVIS spectrum system (Caliper Life Sciences). Serial BL
images displayed in pseudocolors superimposed to a white light image show
tumor growth of two subcutaneous tumors at the left and right back region
(C). BLI signals that arise from the tumors were quantified (B) (gray: left
tumor, black: right tumor) to assess viable tumor volumes.
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