Biomedical Engineering Reference
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Fig. 3 Image of 12 mm in diameter glass coverslips ( a ) coated, from top to bottom, with: PEI
(standard), 10-, 30-, and 60-nm-thick SWCNT-PEG fi lms. Fluorescence images of live hippocam-
pal neurons grown on coverslips coated with PEI coated ( b ) or SWCNT-PEG fi lms of varying
thickness/conductivity ( c , 10-nm thick; d , 30-nm thick; e , 60-nm thick). Arrows indicate growth
cones. ( b-e ) Scale bar, 20 m m. ( f ) Drawing summarizing the effects of the conductivity of SWCNT
substrates on neurite outgrowth, growth cones, and body size. The neurite outgrowth was signifi -
cantly greater in neurons grown on the 10-nm thick SWCNT-PEG fi lms. The average area of the
neuron cell body grown on the 30-nm thick SWCNT fi lms was enlarged. Neurons grown on the
PEI had a signifi cantly higher number of growth cones than those grown on 10- and 30-nm SWCNT
fi lms, but not higher than those grown on 60-nm SWCNT fi lms. Conductivity of each fi lm is listed
in S/cm; PEI is not conductive. Modifi ed from (Malarkey et al. 2009 )
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