Biomedical Engineering Reference
In-Depth Information
Fig. 5
Derivation of the average number of functional antibodies on both covalently conjugated
and streptavidin-biotin-conjugated quantum dots based on measurements of the bound density for
different concentrations. (
a
,
b
) Fitted linear log control curves (ln
y
=
ax
•
b
) for known volumes
of unconjugated IgG antibody band densities in SDS-PAGE gels. Note that the data for each gel
was fi tted with its own curve in order to control for intergel variability. Each symbol represents a
different gel (
n
= 6 gels for covalently conjugated IgG conditions containing a total of 32 unconjugated
IgG controls and 13 IgG-quantum dot complexes, and 7 gels for streptavidin-biotin, IgG-quantum
dot complexes containing a total of 35 unconjugated IgG controls and 28 IgG-quantum dot com-
plexes). (
c
,
d
) Corresponding derived volumes from SDS-PAGE band densities for conjugated and
streptavidin-biotin-conjugated antibody-quantum dot complexes using the curves plotted in pan-
els (
a
) and (
b
), respectively. (
e
) Calculated values for the average number of antibodies conjugated
to quantum dots for both conditions based on the derived measurements of functional antibody
volumes (* and **
p
< 0.01). Reproduced from Pathak et al. (
2007
)
