Biomedical Engineering Reference
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Fig. 4 Separation of IgG antibodies into fragments using SDS-PAGE and membrane transfer
under different experimental and control conditions. ( a ) Covalently conjugated IgG to quantum
dots via an SMCC linker and controls. ( b ) Colloidal nonspecifi c stain for proteins in gels for the
direct conjugation method. ( c ) Biotinylated IgG bound to streptavidin-coated quantum dots and
controls. Reproduced from Pathak et al. ( 2007 )
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