Life Cycle Risks and Impacts of Nanotechnologies - Nanotechnology and Human Health - page 258

TABLE 11.11
Existing In Vivo Animal Studies on Nanosilver

Exposure
Routes

Exposed
Animals

Experiment
Time NP Size Type of Test Endpoint Observed Doses a References

21, 22, 49, 50 nm Dermal Subacute Pig 14 days Morphological alterations 0.34, 3.4, 34 μg/mL Samberg et al. 159

<100 nm Dermal Acute and

subchronic

Guinea
pigs

14 days,
13 weeks

Toxicity, biodistribution 100, 1000, 10,000

μg/mL

Korani et al. 160

13 nm Ingestion Acute Mice 4 days Toxicity, biodistribution,

gene expression

2.5 g Cha et al. 161

60 nm Ingestion Subchronic Rats 29 days Toxicity, biodistribution,

hematology, biochemistry

30, 300, 1000 mg/
kg

Kim et al. 162

22, 42, 71 nm Ingestion Subacute Mice 14, 28 days Toxicity, biodistribution,

hematology, biochemistry

0.25, 0.5, 1 mg/kg Park et al. 163

14 nm Ingestion Acute and

subacute

Rats 14, 28 days Toxicity, biodistribution,

hematology, biochemistry

2.25, 4.5, 9 mg/kg
bw/day

Hadrup et al. 164

56 nm Ingestion Subchronic Rats 91 days Toxicity, biodistribution,

hematology, biochemistry

30, 125, 500 mg/
kg/day

Kim et al. 165

14 nm Ingestion Subchronic Rats 29 days Toxicity, biodistribution 12.6 mg/kg/day

(incl. 1 mg/kg/day
of Ag + )

Loeschner et al. 166

13-15 nm Inhalation Subchronic Rats 29 days Toxicity, biodistribution,

hematology, biochemistry

0.5, 3.5, 61 μg/m 3 Ji et al. 167

22 nm Inhalation Subchronic Mice 2, 4 weeks Gene expression,

morphology

Lee et al. 168

4271 μg/m 3

18-19 nm Inhalation Subchronic Rats 13 weeks Toxicity, biodistribution,

hematology, biochemistry

49, 133, 515 μg/m 3 Sung et al. 169,170

15 nm Inhalation Acute Rats 6 h, 30 h, 4.25

days, 7.25 days

Biodistribution 133 μg/m 3 Takenaka et al. 171

12-15 nm Inhalation Subchronic Rats 28 days Nasal respiratory mucosa 0.5, 3.5, 61 μg/m 3 Hyun et al. 172

18 nm Inhalation Subchronic Rats 91 days Genotoxicity 49, 133, 515 μg/m 3 Kim et al. 173