Biomedical Engineering Reference
In-Depth Information
FIGURE 5.2 Alizarin red (bone) and Alcian blue (cartilage) stained cervical
skeletal regions of fetuses from control (a, c, e) and methanol treated (b, d, f)
CD-1 mice. Pregnant mice were treated with 4 or 5 g/kg by oral gavage on
gestation day 7, and fetuses were examined on gestation day 17. Note splits
and fusion of the atlas and axis (arrow in b) and unilateral displacement (to C5)
of the tubercular anterior (TA) in the ventral view of the treated fetus in d
(compare to the control in c, where both TAs are on C6). Also, compare
disarticulated cervical vertebrae from a control fetus (e) to those from a
methanol-treated fetus (f). TAs (asterisks) on C6 in the control are on C5 in the
treated fetus, and C7 from the treated fetus has a full rib attached to it. Source:
From Connelly and Rogers (1997).
in the effects of methanol and ethanol, Rogers et al. (2004) gave
pregnant female C57BL/6J mice two intraperitoneal (i.p.) injections
of methanol on GD 7, the most sensitive day for induction of cranio-
facial malformations by ethanol. The injections were given 4 hours
apart to provide a total dosage of 0, 3.4, and 4.9 g/kg. Some pregnant
